Biological Imaging

In the life and biomedical sciences the form and function of organisms, particularly at the cellular and subcellular levels, is often studied through imaging and microscopy approaches. This module informs about the concepts of imaging and microscopy; the importance of resolution and its limits; optical components; and microscope design. Students will gain understanding of widefield microscopy, laser scanning confocal microscopy, spinning disk confocal microscopy; super-resolution microscopy, and automated microscopy. The module will also detail various imaging techniques used to quantify the mobility and interactions of molecules in cells, including time-lapse microscopy, FRAP, FRET, FCS and split fluorescent proteins. Cell culture and types of cells available, treatment of cells in live cell imaging experiments, use of media, buffers, climate control systems, and issues relating to photo-toxicity will also be explored. Consideration will also be given to fluorescent proteins, fixation methods, use of antibodies and stains, immunofluorescence, and mounting media. A basic introduction to the analysis of biological images will also be given. This module also provides practical exposure to concepts in imaging through lab-based workshops.